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KMID : 1134820080370091114
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Journal of the Korean Society of Food Science and Nutrition
2008 Volume.37 No. 9 p.1114 ~ p.1119
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Effect of Epigallocatechin Gallate on Apoptosis in MDA-MB-231 Human Breast Cancer Cells
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Hong Eun-Jung
Kim Woo-Kyung
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Abstract
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Among the numerous polyphenols isolated from green tea, epigallocatechin gallate (EGCG) is a predominate and is considered to be a major therapeutic agent. To elucidate the mechanical insights of anti-tumor effect, EGCG was applied to human breast cancer MDA-MB-231 cells. We investigated the effect of EGCG on protein and mRNA expression of proteins related to cell apoptosis in MDA-MB-231 human breast cancer cell lines. We also identified caspase-3 activity. We cultured MDA-MB-231 cells in the presence of 0, 5, 10, and 20 μM of EGCG. Protein and mRNA expression of bcl-2 were decreased dose-dependently in cells treated with EGCG. However, protein and mRNA expression of bax were increased (p＜0.05). Caspase-3 activities were increased dose-dependently in cells treated with EGCG. These results suggest that EGCG induces cell apoptosis by increase of caspase activity through decreasing of protein and mRNA expression of bcl-2 and increasing of protein and mRNA expression of bax.
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KEYWORD
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epigallocatechin gallate, breast cancer, apoptosis, bcl-2 family, caspase
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